Permadeath


 * When the player's body is desecrated, the run ends and Homunculus (humanoid creature) is sent back to the Prisoners' Quarters, where it finds a new body and starts a new run. All cells and blueprints that have not been turned in to The Collector are lost, as well as level progress and currently equipped weapons, skills, and amulets. Additionally, some amount of gold is lost, depending on the player's current Gold Recovery mutations upgrade level ; the remaining gold can be found in a dead man's purse next to the player's new body.


 * However, after successfully completing the run, if player had any unspent cells, these are not lost and all off them can be found next to gold purse from previous run. This leftover cells from previous game can be used in the next run, but this will be lost, if player will die before reaching biome exit and spending them at The Collector place, or don't pick them up at all.


 * The layouts of the biomes are procedurally generated each run.

Trivia
It is possible for the player to die without their body being desecrated, by picking the Ygdar Orus Li Ox mutation (but it won't prevent death by curse).