Bosses

Keepers are the bosses in Dead Cells. Each keeper has their own biome, where the player must defeat them to progress. Currently the player can defeat only three of the five bosses per run on 0 to 4 BSC difficulty levels. On 5 BSC difficulty the player can defeat four of the six keepers.

There are currently seven keepers in the game: The Concierge, Conjonctivius, The Time Keeper, The Giant, The Hand of the King, and The Collector. The seventh, Mama Tick, is only available with the Bad Seed DLC.

First bosses
As the first boss, the player may find one of these two, depending on the path that they take.

The Concierge
The first boss encountered in the game, as it requires only the Vine Rune to be reached. Found in the Black Bridge. Must be defeated to reach the Stilt Village.

Conjonctivius
Can be reached by going through the Toxic Sewers, then the Ancient Sewers, to the Insufferable Crypt.

The player must have absorbed both the Vine Rune and the Ram Rune in order to take this path.

Mama Tick
Can be reached if the player owns the Bad Seed DLC. It is only accessible by going through the Morass of the Banished and traveling to the Nest. The player does not need any specific runes coming from the Morass of the Banished, for the Nest is the only exit.

The Time Keeper
Found in the Clock Room. Can be reached through either the Clock Tower or the Forgotten Sepulcher.

The Giant
Found in the Guardian's Haven. Must be reached through the Cavern on the first meeting, can be reached via a 2 BSC door in Forgotten Sepulcher afterwards.

The Hand of the King
The final boss of the game on 4 BSC and less. Found in the Throne Room. He is reached through High Peak Castle or Guardian's Haven.

The Collector
The true final boss of the game. Found in the Observatory, which can only be reached with 5 BSC active, after the Throne Room and the Astrolab.