Time, Killstreak & No-Hit Doors

Timed doors appear in most biomes. If the door is not reached and opened within a set amount of time in a run, they will be locked and unopenable. Time spent in the beginning area of The Prisoners' Cell, The Collector's Room and in shops does not count towards timed doors. Behind each door is some gems and jewels that become gold when picked up as well as some cells. Sometimes there also is a scroll.

The Prisoners' Cell
No timed door.

The Promenade of the Condemned
Door closes at 3 minutes, 15 seconds.

The Toxic Sewers
Door closes at 3 minutes, 30 seconds.

The Ramparts
Door closes at 6 minutes.

Old Sewers
No timed door.

Ossuary
Door closes at 5 minutes, 30 seconds.

The Prison Depths
???

The Black Bridge
No timed door.

The Insufferable Crypt
No timed door.

Fog Fjord
There are two doors in this biome: One in the collector's room right before it that closes at 10 minutes, 30 seconds and another one inside a building that closes at 15 minutes.

Graveyard
???

Slumbering Sanctuary
???

Forgotten Sepulchere
Door closes at 10 minutes, 30 seconds.

The Clock Tower
???

Clock Room
No timed door.